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KMID : 0357119950170010027
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Korean Journal of Immunology
1995 Volume.17 No. 1 p.27 ~ p.32
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Antitumor Effects of Lymphokine-Activated Killer(LAK) Cells on Cisplatin and Adriamycin Resistant Cancer Cells
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홍원선
민영일/홍석일/손영숙/윤연숙
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Abstract
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Antitumor effects of lymphokine-activated killer(LAK0 cells on the acquired resistance to cisplatin and adriamycin were investigated using 51Cr release assay in human stomach adencarcinoma cell line (MKN-45), human pulmonary ade
ocarcinoma cell line(PC-14) and their sublines resistant to cisplatin (MKN/CDP and PC/CDDP) and adriamycin(MKN/ADM and PC/ADM). LAK cells were generated by incubating peripheral blood lyumphocytes(PBL) obtained from five healthy volunteers with
1×10E3
U/ml of recombinant human interleukin-2 for five days. The cytotoxicity of LAK cells against K562 was significantly higher than that of PBL at E:T ratios of 10:1(p <0.05).LAK activities against cisplatin resistant sublines(MKN/CDDPD and PC/CDDP)
and
adriamycin resitant sublines (MKN/ADM and PC/ADM) were similar to those against their corresponding parent cell lines, MKN-45 and PC-14, respectively. These results demonstrating the similar susceptibility against LAK cells between resistant
cells
and
parent cells suggest that the immunotherapy with LAK cells may be useful for the treatment of drug-resistant cancer.
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KEYWORD
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